A group calling itself the Iranian Cyber Army has hacked Twitter and an Iranian opposition website, replacing it with an anti-American message.
Traffic to the social networking website was redirected for nearly two hours on Thursday night.
The opposition website mowjcamp.org remained disrupted on Friday.
The opposition in Iran have used the websites to publicise protests and accuse the government of rigging elections in June.
"This site has been hacked by the Iranian Cyber Army," the message read.
The message, written in confused English, said the hackers had turned the tables on the US, who they criticise for sanctions on Iran.
"USA think they controlling and managing internet by their access, but they don't, we control and manage internet by our power," the message continued.
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